Extremophilic Actinomycetes, Distributed in Various Types of Soils of Georgia and their Protease Activity

Protease activity of actinomycetes isolated from various soil types of Georgia has been studied. Protease activity strains have been identified among mesophilic and extremophilic actinomycetes. Some strains of mesophilic, alkaliphilic and halophilic actinomycetes are characterized by alkaline protease activity. Their pH optimum for enzyme activity is 10-11. Strains of mesophilic actinomycetes: Streptomyces glaucus 71MD, Streptomyces levoris 114D, Streptomyces coelicolor 774 are of great interest. Their enzymatic activity at pH-11 corresponded 2.5unit/ml, 1.3unit/ml, 2.3unit/ ml, respectively. Alkaliphilic actinomycetes strains Streptomyces streptomycini 204A and Nocardia paloris 206A with protease activity 0.85unit/ml and 1.1unit/ml respectively are also important strains. Optimum pH of enzyme activity is 10. Halophilic strain spp. 387H reveals maximum enzyme activity at pH-10 – 1.2unit/ml. © 2010 Bull. Georg. Natl. Acad. Sci.